Oklahoma State University


Dr. Jeanmarie Verchot-Lubicz
209E NRC, 744-7895
verchot.lubicz@okstate.edu 		Dr. Haobo Jiang
329K NRC, 744-9400
haobo.jiang@okstate.edu


Lecture notes:


History of biotechnology
Cloning
Gene Expression and Regulation
Agrobacterium
Plant Transformation
Analysis of gene expression at various levels
Green fluorescent protein (GFP)
DNA Synthesis and its Applications
Transcription and its Regulation
Translation: Protein Synthesis
Expression and Purification of Recombinant Proteins
Polymerase Chain Reaction (PCR): Techniques and Applications
Mass Spectrometry and Proteomics

Laboratory notes:


Lab 1 - Plasmid DNA purification
Lab 2 - Micropipetting
Lab 3 - Restriction Enzyme Cleavage of DNA
Lab 4 - Agrobacterium
Lab 5 - Vectors & cloning tools
Lab 6 - Proteomics

Experiment sets:


Overview of the experiments
Session I
Session II
Session III
Session IV
Session V
Session VI
Northern-Western Blot Analysis
Session VII

Additional readings:


Preuss
Heslop-Harrison et al.
Jin et al.

Weblinks for Agrobacterium and GM crops:


http://www.youtube.com/watch?v=ehwb8Wx2kZ0&feature=related
http://www.youtube.com/watch?v=gvUkorD0kgA&feature=related
http://www.youtube.com/watch?v=_22N8WTPFEk&feature=fvw
http://www.youtube.com/watch?v=L7qnY_GqytM
http://www.youtube.com/watch?v=crmj7A1zQ2k&feature=related
http://www.youtube.com/watch?v=x_hDBqrW-OQ
http://www.youtube.com/watch?v=n9xpKx03g9M&feature=related
http://www.youtube.com/watch?v=uk130lyAVBM&feature=related
http://www.youtube.com/watch?v=ztWWqugczm8&feature=related
http://www.youtube.com/watch?v=04rz1lx3eok&feature=related
http://www.youtube.com/watch?v=DPGcryNfqIo
http://www.ppws.vt.edu/~sforza/prokaryote.html

Resources:


Cloning
Cloning Vectors
Flybase - Common cloning vectors and constructs
Greenomes
Restriction Enzymes
Restriction Enzymes Background Paper

Movies:


Movie - Intro plant genomes - (195 MB, right click save, open in Windows Media Player)
Movie - Cotton plant genomes - (168 MB, right click save, open in Windows Media Player)
Movie - Corn plant genomes - (175 MB, right click save, open in Windows Media Player)
Movie - Potato plant genomes - (254 MB, right click save, open in Windows Media Player)
PBS Nova - Meet the Decoders
PBS Nova - Journey into DNA
PBS Nova - Genome
HGSC - Honey Bee Genome Project
NHGRI - Human Genome Project
NHGRI - The Large-Scale Genome Sequencing Program
NHGRI - Understanding the Human Genome Project
Science Mag - The Macaque Genome
Nature - The honeybee genome
Science Daily - Doggy Genes

Other Movies on YouTube:


DNA Fingerprinting (Britannica.com)
DNA Fingerprinting
Electrophoresis
Gel Electrophoresis
Gel Electrophoresis - Agarose
Plasmid Cloning
Protein Structure
Restrict Enzymes
RNA to Protein Synthesis
Division of Agricultural Sciences and Natural Resources