Entomology and Plant Pathology

Dr. Haobo Jiang, Professor

haobo new
B.S., East China University of Chem Tech, 1985

Ph.D., Kansas State University, 1994
haobo.jiang@okstate.edu

 

Links:

A list of conserved and novel microRNAs from Manduca sexta

Manduca sexta CIFH contigs (.rtf file format) or (.pdf file format) 

Manduca sexta EST contigs of hemolymph proteins involved in immune responses

Serine Protease (SP) and Serine Protease Homologous (SPH) Proteins in Drosophila melanogaster

A multiple sequence alignment of the glucanase-like proteins in invertebrates

Sequence alignment of the catalytic domains of Manduca sexta HPs and other arthropod serine proteinases with known functions

Teaching:

ENTO/PLP 5623     Advanced Biotechnology Methods
ENTO 5833      Insect Molecular Biology

Lab members, students, and collaborators:

Yang Wang, Senior Research Specialist
Native of Shanghai, China 1986  B.S., East China University of Chemical Technology, Biochemistry
1994  M.S., Kansas State University, Biochemistry

Fan Yang, Graduate Research Assistant
Native of Jiangsu, China
2004 B.S., East China University of Science and Technology, Food Science and Technology
2007 M.S., East China University of Science and Technology, Microbial and Biochemical Pharmacology

Xiufeng (Stephen) Zhang, Graduate Research Assistant
Native of Henan, China
2009 B.S., University of Science and Technology of China, Molecular Biology and Cell Biology

Yinxia (Alicia Hu), Graduate Research Assistant
Native of Zhejiang, China
2009 B.S., University of Science and Technology of China, Biotechnology

Shuguang (Leo) Zhang, Graduate Research Assistant
Native of Hebei, China
2009 B.S., University of Science and Technology of China, Molecular Biology and Cell Biology

Ramesh T. Gunaratna, Graduate Research Assistant
Native of Colombo, Sri Lanka
2009 B.S., University of Colombo, Bioinformatics

Xiaolong Cao, Graduate Research Assistant
Native of Anhui, China
2011 B.S., University of Science and Technology of China, Molecular Biology and Cell Biology

Yan He, Graduate Research Assistant
Native of Hunan, China
2011 B.S., University of Science and Technology of China, Systems Biology

Current research:

  • Extracellular serine proteinase cascades have evolved in vertebrates and invertebrates to mediate rapid defense reactions to wounding and infection. We have started to investigate such enzyme system and its regulation by proteinase inhibitors in a biochemical model insect, Manduca sexta. In collaboration with Dr. Kanost's group at Kansas State University, we are investigating the prophenoloxidase-activating pathway, a defense mechanism triggered by invading pathogens. Active phenoloxidase catalyzed the formation of reactive intermediates that are utilized to kill pathogens, seal wounds, and encapsulate parasites. 
  • Prophenoloxidase is activated via limited proteolysis. We have purified three different prophenoloxidase-activating proteinases (PAPs) from M. sexta and examined their expression patterns. Transcription of these clip-domain serine proteinases is regulated in tissue-, development-, and defense-specific manners. 
  • We have isolated cDNA clones for 22 different hemolymph proteinases, some of which are members of the serine proteinase system that coordinates the immune responses in M. sexta hemolymph. A modular protein, designated M. sexta HP14, interacts with ß-1,3-glucan or peptidoglycan, autoactivates, and triggers the prophenoloxidase-activating pathway. 
  • This proteinase pathway is negatively regulated by serine proteinase inhibitors of the serpin superfamily. M. sexta serpin-1 variants, serpin-3, -4, -5, and -6 form stable proteinase-inhibitor complexes with one or more of the pathway components. Since molecular probes (e.g., cDNAs and antisera for HPs and serpins) are available, we are attempting to elucidate the prophenoloxidase-activating pathway, its relationships with other proteinase-mediated immune responses, and inhibitory regulation by serpins.

Laboratory Specialization:

  • hemolymph proteinases involved in insect immune responses 
  • protein purification by HPLC/FPLC 
  • characterization of hemolymph proteins 
  • serine proteinase inhibitors of the serpin superfamily 
  • expression of proteins in E. coli and baculovirus expression systems 
  • molecular cloning of cDNAs and genes 
  • transcriptional regulation of immune genes

Invited Symposium Presentations:

1996.05   International Symposium on the Chemistry and Biology of Serpins.
1996.07   Gordon Research Conference ("Proteolytic Enzymes and their Inhibitors")
2000.08   XXI International Congress of Entomology
2000.10   Annual Meeting of the Arbeitskreis Comparative Immunology, Germany
2003.07   Symposium on the Molecular Basis of the Recognition of Foreignness in Innate Immunity, Sapporo, Japan
2004.09   International Symposium on protein science in arthropods, Tsukuba, Japan
2005.12   The 4th Symposium of Biological and Medical Sciences at TMMU, Chongqing, China

Publications:

Click here for a complete PDF list of my publications.

 

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